DNA Barcoding and Morphological Characters of Two Trevally Fish species (Caranx Spp.) Collected from Youtefa Bay, Papua, Indonesia

Trevally fish (Caranx spp,) or Kuwe (local name) are small pelagic that mostly caught in the waters of Youtefa Bay. There two types difficult to distinguish due morphological similarities. These have no scientific species identification so they several different local names. Accordingly, it is necessary identify and find out proper naming fish. DNA barcoding a technique used speed up simplify process identifying organisms. Hence, purpose this research was name Bay waters, as well kinship between these by applying technique. Nineteen were sampled at Bay, Jayapura, Papua July 2022. Samples studied morphologically, then carried directly Integrated Laboratory Diponegoro University for molecular studies. extraction, amplification, sequencing further validate species’ identification. The results showed initial assessment specimens using taxonomic keys, 10 identified Caranx sexfasciatus 9 tille. relationship length weight C. tille resulted b = 2.5754 with R2 0.8564 1.7119 0.6705, respectively. All identifications confirmed correct based on BLASTn results. BLAST analysis demonstrated samples closely related (99%-100% similarity) similar (97%- 99%). genetic distance individuals has value ranging from 0% - 0.2%, phylogenetic tree exposed included clades. This study revealed there (C. tille) Papua, Indonesia.